Antibodies used in this study were ACTB Sigma Aldrich, A5441, VCL vinculin Sigma Aldrich, V4505, MAP1LC3B Cell Signaling Technology, 2775, SQSTM1 Cell Signaling Technology, 5114, SQSTM1 Enzo Life Sciences, BML PW9860, TOMM20 Santa Cruz Biotechnology, SC 11, 415, phospho EIF4EBP1 Cell Signaling Technology, 9451, EIF4EBP1 Cell Signaling Technology, 9644, phospho RPS6KB Cell Signaling Technology, 9205, RPS6KB Santa Cruz Biotechnology, SC 230, phospho RPS6 Cell Signaling Technology, 5364, RPS6 Cell Signaling Technology, 2317, PECAM1 CD31 PacBlue Biolegend, 102, 422, PTPRC CD45 PacBlue Biolegend, 103, 126, LY76 Ter119 PacBlue Biolegend, 116, 232 and CD24 Alexa Fluor 647 Biolegend, 101, 818 cialis price The systolic blood pressure of rats drinking regular water was not changed over the 4 week treatment period 118