The lung tumor tissues from xenografted mice were sectioned, and the slides were stained with anti Twist1 primary antibody at 4 C overnight, followed by incubation with HRP labeled goat anti rabbit secondary antibody for 1 h at room temperature, and stained with 3, 3 diaminobenzidine DAB solution <a href=https://buylasixon.com/>lasix blood thinner</a> van Santbrink EJ, Fauser BC