Then, 4 hydroxytamoxifen 4 OHT Sigma Aldrich was dissolved in ethanol and added to the culture media to produce a range of drug concentrations 1 80 ОM, and the cells were incubated with the drug for an additional 48 hours clomid twins A fully functioning testis normally achieved by the age of 16 years has the capacity to produce over 200 million sperm each day